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. Author manuscript; available in PMC: 2022 May 19.
Published in final edited form as: Methods Mol Biol. 2018;1715:191–205. doi: 10.1007/978-1-4939-7522-8_13

Table 3.

Primer sequences for PCR

Primers Sequence (5′ → 3′)
T7-sgRNA1-foward TTAATACGACTCACTATAGGGCCACTTTCTGCTACTTAGGT
T7-sgRNA2-foward TTAATACGACTCACTATAGGGCTCCAGGCCTTGGCCTGTAC
T7-sgRNA3-foward TTAATACGACTCACTATAGGGAGGGCCCAGTGATCAGCCGG
T7-sgRNA4-foward TTAATACGACTCACTATAGGGCCAACCTAAGTAGCAGAAAG
sgRNA-reverse AAAAGCACCGACTCGGTG
RD1-check-F CAAGAAGGCAGTAGGATTCCG
RD1-check-R TTGTCTTGCCTGCTTCTCATC
#10 ATGTACCGCCAGCGCAATGG
#JS610 CCCCGCCTTCTCAACAACCTGGGACGGGAG
#80 CTCTGTTTCTCTCCTGATACG
#81 ACCTGCATGTGAACCCAGTATT