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. 2022 May 19;18(5):e1010485. doi: 10.1371/journal.ppat.1010485

Fig 1. MAVS KO mice with IFN-I signaling blocked are protected against lethal infection by CCHFV.

Fig 1

A. C57BL/6 mice or MAVS KO mice (n = 5/group) were infected with CCHFV and survival and weight loss were monitored and plotted using Prism software. Only WT mice were treated with MAb-5A3 24h after infection to block IFN-I. IFN-I was not blocked by antibody in the MAVS KO mice. B. B6.129 or MAVS KO mice (n = 6/group) were infected with CCHFV and both groups treated 24 h post-infection were treated with mAb-5A3. Survival and weight loss were monitored for 20 days and plotted using Prism software. Survival significance was determined by log-rank analysis; ***p<0.0001. C. Viral RNA in serum and liver was examined on day 4, 10 and 15 by RT-qPCR (n = 3 per group). Mean titers +/- SEM of the estimated PFUs (PFUe) were graphed. The dashed black line denotes limit of detection. Statistical significance was determined by one-way ANOVA; *p<0.05. D. Monocyte chemoattractants and inflammatory cytokines were measured from the serum (n = 3 per group) of CCHFV infected mice on day 4 using a multiplex system. Statistical significance compared to uninfected controls was determined by one-way ANOVA; *p<0.05, ***p<0.001. E. C57BL/6, MDA5 KO or MAVS KO mice (n = 8 per group) were infected with CCHFV and 24 h post-infection were treated with MAb-5A3. Survival and weight loss were monitored for 20 days. Significance determined by log-rank analysis (p<0.0001).