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. 2022 May 19;18(5):e1010485. doi: 10.1371/journal.ppat.1010485

Fig 5. Liver injury is reduced in CCHFV infected mice missing TNFA-R signaling.

Fig 5

A Representative H&E staining of livers from WT (C57BL/6) and TNFA-R DBL KO mice infected with CCHFV in the presence or absence of IFN-I blockade harvested on Day 4. Livers have multifocal areas of random, lytic and single cell hepatocyte necrosis with accompanying inflammation which are larger in WT mice compared to the smaller, more discrete areas of necrosis in TNFA-R DBL KO mice. B. Pathology scores were plotted for the indicated lesions or ISH staining (n = 3/group/time point). Statistical significance of day 4 and day 10 infected, TNFA-R DBL KO mice compared to infected controls was determined by one-way ANOVA; **p<0.05 or NS; not significant. C. Liver sections from day 4 infected or uninfected WT (C57BL/6) or TNFA-R DBL KO mice were stained with anti-CLEC4F (red) and anti-CCHFV N protein (green) antibodies. Cell nuclei were stained with DAPI. Arrows point to CLEC4F+ cells and arrow heads point to infected CLEC4F+ cells.