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. 2022 May 9;18(5):e1010190. doi: 10.1371/journal.pgen.1010190

Fig 2. Linear deleted subgenomic fragments do not accumulate with age in Mgme1-/- mice.

Fig 2

(A) Southern blot quantification of linear deletion fragment signal in ratio to total mtDNA signal in liver, kidney,heart, skeletal muscle and brain of Mgme1-/- mice at 10 and 50 weeks of age. Error bars represent SD. (B) De novo DNA synthesis in heart mitochondria isolated from control (+/+) and Mgme1 knockout (-/-) mice. Mitochondria were pulse labeled (p) for 2 h and the chase was performed for 1 and 3 h. SDHA and VDAC levels on western blot represent loading control for the input mitochondria. (C) Quantification of full length mtDNA (left panel), total mtDNA (middle panel) and 7S DNA (right panel) in wild-type and MGME1 knock-out heart tissue of young (10 weeks) and old (55 weeks) animals. Values are given as mean ± SD. * P ≤ 0.05, T-test. (D) As (C) but in kidney tissue.