Fig 2. Generation of Mdm2^L466A allele.

(A) A diagram depicting the strategy for creating the Mdm2^L466A mutant allele is shown. A conventional targeting vector, sgRNA (in green) and anti-sense template DNA sequences including Mdm2^L466A mutations are shown. (B) Genotyping results of wild type Mdm2 and Mdm2^L466A alleles by PCR and DNA sequencing using tail DNA from mouse pups. The wild-type Mdm2 amplicon (318bp), the Mdm2^L466A amplicon (198bp). (C) The nucleotide changes for codon L466 in DNA sequencing chromatograms are shown.