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. Author manuscript; available in PMC: 2022 May 19.
Published in final edited form as: J Virol Methods. 2020 Apr 14;280:113865. doi: 10.1016/j.jviromet.2020.113865

Fig. 6. Estimated cost per sample for performing next-generation sequencing based on kits used for sequencing and the level of multiplexing.

Fig. 6.

From left to right, each block represents the number of samples multiplexed in a single run. Individual bars correspond to the library preparation and sequencing kit used. The number above each bar indicates the estimated cost per sample. The Ion PGM and S5 calculations are only performed out to multiplexing levels of 24 samples, as the KAPA DNA library kit currently only makes 24 unique indices. Calculations include the cost of reagents, kits and consumables from sample pretreatment through sequencing (Fig. 1). All preparations compared in this figure are based on using half-reactions for library preparation.