TABLE 1.
Bacterial strains and plasmid used
| Strain or plasmid | Relevant characteristics | Source and/or reference |
|---|---|---|
| E. coli strains | ||
| W3110 | F− IN(rrnD-rrnE)1 | B. Bachmann |
| BL21(DE3) | hsdS gal (λcIts857 ind1 Sam7 nin5 lacUV5-T7 gene 1) | Lab collection |
| HB101 | supE44 hsdS20(rB−mB−) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 | Lab collection |
| MC4100 | F−araD139 Δ(argF-lac)U169 rpsL relA deoC ptsF rbsR flbD | B. Bachmann |
| RR1 | supE44 hsdS20(rB−mB−) ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 | Lab collection |
| BW1677 | pta::TnphoA′-3 | B. Wanner |
| CP993 | OW1 Φ(pta-1::Tn10-lacZ) | C. Park (23) |
| NZN117 | LCB320 ldhA::Kan | D. P. Clark (5) |
| KJE103 | W3110 ppc::cat | Lab collection |
| JP201 | RR1 pta::TnphoA′-3 | P1 (BW16777) × RR1 |
| JP202 | RR1 Φ(pta-1::Tn10-lacZ) | P1 (CP993) × RR1 |
| JP203 | RR1 pta::TnphoA′-3 ppc::cat1 | P1 (KJE103) × JP201 |
| JP204 | RR1 Φ(pta-1::Tn10-lacZ) ldhA::Kan | P1 (NZN117) × JP202 |
| JP205 | RR1 Φ(pta-1::Tn10-lacZ) ldhA::Kan ppc::cat | P1 (KJE103) × JP204 |
| Plasmid pLS65 | l-LDH gene from L. casei | M. Y. Park (16) |