TABLE 3.
Fermentation balances of the E. coli RR1 pta ldhA mutant and its recombinant harboring foreign l-LDH from L. caseia
| Carbon source or product | Relative amt consumed or produced by:
|
|
|---|---|---|
| JP204 | JP204(pLS65) | |
| Glucose (carbon source) | 1.00 (12.2)b | 1.00 (12.5) |
| Products | ||
| Acetate | 0.12c | 0.04 |
| Ethanol | 0.42 | 0.24 |
| Lactate | 0.00 | 0.72 |
| Succinate | 0.25 | 0.20 |
| Pyruvate | 0.36 | 0.00 |
| Formate | 0.44 | 0.21 |
a
Fermentation balances were determined relative to a value of 100 per dimethyl sulfone, which was the internal standard used in all NMR experiments performed in this study. All measurements were obtained in triplicate and were independent. The standard deviations were less than 10%.
b
The numbers in parentheses are millimoles of glucose consumed.
c
Millimole of product (as quantified by NMR) per millimole of glucose consumed.