. 2022 May 4;13:820431. doi: 10.3389/fmicb.2022.820431

Table 1.

Species-specific genes and primers for PCR and qPCR identification of P. aeruginosa.

Species	Name of target genes	^*Gene location	Encoded protein	Primer set name	Sequences (5′-3′)	Product size (bp)	For PCR or qPCR assay
P. aeruginosa	UCBPP-PA14_00095 (group_98983)	110,675–110,959	Hypothetical protein	PA1	CTCCGTGGAAAAGCAGTTG	169	PCR
				PA1	GCGTATGCCGACGTAGAAT	169	PCR
				PA12	AATGCGGGATGCTGCTCT	138	qPCR
				PA12	GGTCGGTCTCCTCGAACTCTT	138	qPCR
	UCBPP-PA14_03237 (phzA2)	3,561,244–3,561,732	Phenazine biosynthesis protein PhzA2	PA2	GTTTACCGACAACCTGGAA	325	PCR
				PA2	GCAATAGCCCTGCGGATAC	325	PCR
				PA13	CAACTGGACCACGGAAAGC	126	qPCR
				PA13	GTCTCGAAGATCCGCACGT	126	qPCR
	UCBPP-PA14_04976 (group_75393)	5,434,538–5,434,912	Hypothetical protein	PA3	ATGGACAGGGACGCATTGA	263	PCR
				PA3	CGAGGGACGAAGGTAAGGA	263	PCR
				PA14	CGGTACAGGTCGGCACG	109	qPCR
				PA14	CGAGGGACGAAGGTAAGGA	109	qPCR
	UCBPP-PA14_03627 (group_88276)	3,973,724–3,974,497	Hypothetical protein	PA4	GACTCTACCCTCCCTGACTT	132	PCR
				PA4	TCCATCACCGAGAAGC	132	PCR
				PA15	TACGCGGTCAGCCATCAA	104	qPCR
				PA15	CAGCTCACTGCCGTTTCC	104	qPCR

Reference strain is P. aeruginosa UCBPP-PA14. The reference gene is GCA_000014625.1_ASM1462v1.