Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Mus musculus) | B6;129S-Tac1tm1.1(cre)Hze/J | The Jackson Laboratory | JAX: 021877 | Tac1Cre mouse |
| Genetic reagent (Mus musculus) | B6(Cg)-Crhtm1(cre)Zjh/J | The Jackson Laboratory | JAX: 012704 | CrhCre mouse |
| Genetic reagent (Mus musculus) | B6.Cg-Calcatm1.1(cre/EGFP)Rpa/J | The Jackson Laboratory | JAX: 033168 | CalcaCre mouse |
| Genetic reagent (Dependoparvovirus) | AAV9-hSyn-DIO-mCherry | Addgene | #50,459 | Adeno-associated viral vector |
| Genetic reagent (Dependoparvovirus) | AAV9-CAG-Flex-GCaMP6s | Addgene | #100,842 | Adeno-associated viral vector |
| Genetic reagent (Dependoparvovirus) | AAV9-hSyn-DIO-hM3Dq-mCherry | Addgene | #44,361 | Adeno-associated viral vector |
| Genetic reagent (Dependoparvovirus) | AAV9-hSyn-DIO-hM4Di-mCherry | Addgene | #44,362 | Adeno-associated viral vector |
| Genetic reagent (Dependoparvovirus) | AAV5-Ef1a-DIO-ChR2-mCherry | UNC North Carolina Vector Core | #AV4379J | Adeno-associated viral vector |
| Genetic reagent (Dependoparvovirus) | AAV5-Ef1a-Flex-TVA-mCherry | Stanford University Gene Vector and Virus Core | #GVVC-AAV-67 | Adeno-associated viral vector |
| Genetic reagent (Dependoparvovirus) | AAV8-CAG-Flex-RabiesG | Stanford University Gene Vector and Virus Core | #GVVC-AAV-59 | Adeno-associated viral vector |
| Genetic reagent (Rabies lyssavirus) | SAD-ΔG-eGFP | Salk Institute Viral Vector Core | SAD-ΔG-eGFP | Modified rabies viral vector |
| Antibody | anti-Fos (Rabbit polyclonal) | Cell Signaling Technology | #2,250 | (1:1000) |
| Antibody | AlexaFluor 488-conjugated anti-rabbit (Donkey polyclonal) | Jackson ImmunoResearch | #711-545-152 | (1:250) |
| Chemical compound, drug | Amylin | Bachem | #H-9475 | 10 μg/kg |
| Chemical compound, drug | Cholecystokinin | Bachem | #4033010 | 10 μg/kg |
| Chemical compound, drug | Protein YY | Bachem | #H-6042 | 100 μg/kg |
| Chemical compound, drug | Clozapine-N-oxide | Sigma-Aldrich | #C0832 | 0.3 mg/kg |
| Chemical compound, drug | 2,2,2-Tribromoethanol | Sigma-Aldrich | #48,402 | |
| Sequence-based reagent | RNAscope Probe-Mm-Crh | ACDBio | #316,091 | |
| Sequence-based reagent | RNAscope Probe-Mm-Tac1 | ACDBio | #410,351 | |
| Sequence-based reagent | RNAscope Probe-Mm-Tac1-C3 | ACDBio | #410351-C3 | |
| Sequence-based reagent | RNAscope Probe-Mm-Slc17a6-C2 |
ACDBio | #319171-C2 | |
| Sequence-based reagent | RNAscope Probe-Mm-Calb1-C2 | ACDBio | #428431-C2 | |
| Sequence-based reagent | RNAscope Probe-Mm-Calb2-C2 | ACDBio | #313641-C2 | |
| Sequence-based reagent | RNAscope Probe-Mm-Pvalb-C2 | ACDBio | #421931-C2 | |
| Sequence-based reagent | RNAscope Probe-Mm-Fos-C2 | ACDBio | #316921-C2 | (1:10) |
| Commercial assay, kit | RNAscope Multiplex Fluorescent Reagent Kit v2 | ACDBio | #323,100 | |
| Software, algorithm | Fusion Software | Omnitech Electronics | Food intake measurement software | |
| Software, algorithm | Synapse Software | Tucker-Davis Technologies | Fiber photometry measurement software | |
| Software, algorithm | MATLAB | Mathworks | Fiber photometry measurement data analysis | |
| Software, algorithm | Prism 8.0 | GraphPad | Statistical software | |
| Other | Opal 520 | Akoya Biosciences | #FP1487001KT | Histological stain for RNAscope (1:1500) |
| Other | Opal 570 | Akoya Biosciences | #FP1488001KT | Histological stain for RNAscope (1:1500) |
| Other | Opal 690 | Akoya Biosciences | #FP1497001KT | Histological stain for RNAscope (1:1500) |
| Other | DAPI Fluoromount-G | Southern Biotech | #0100–20 | Slide mounting medium with DAPI nuclear stain |
| Other | Fluoromount-G | Southern Biotech | #0100–01 | Slide mounting medium |