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. 2022 May 19;11:e73625. doi: 10.7554/eLife.73625

Key resources table.

Reagent type (species) or resource Designation Source or reference Identifiers Additional information
Strain, strain background (Brucella abortus) Strain 2308 Maintained by the de Figueiredo lab WT strain
Strain, strain background (B. abortus) S19 Maintained by the de Figueiredo lab Smooth vaccine strain
Strain, strain background (B. melitensis) 16M Maintained by the de Figueiredo lab WT strain
Strain, strain background (B. melitensis) 16M ΔVirB2 Maintained by the de Figueiredo lab ΔVirB2 derived from 16M
Strain, strain background (B. melitensis) 16M ΔVjbR Maintained by the de Figueiredo lab ΔvjbR derived from 16M
Strain, strain background (Mouse Hepatitis Virus) A59 Other Gift from Leibowitz lab
Strain, strain background (Lentivirus) LLV-LentiGUIDE-Puro-Bloc1s1.1-gRNA This paper The de Figueiredo lab; see Methods and methods
Strain, strain background (Lentivirus) LLV-LentiGUIDE-Puro-Bloc1s1.2-gRNA This paper The de Figueiredo lab; see Methods and methods
Strain, strain background (Lentivirus) LLV-LentiGUIDE-Puro-Bloc1s1.3-gRNA This paper The de Figueiredo lab; see Methods and methods
Strain, strain background (Lentivirus) LLV-LentiGUIDE-Puro-Bloc1s1.4-gRNA This paper The de Figueiredo lab; see Methods and methods
Strain, strain background (Lentivirus) LLV-LentiGUIDE-Puro-Bloc1s1.5-gRNA This paper The de Figueiredo lab; see Methods and methods
Strain, strain background (Lentivirus) LLV-LentiGUIDE-Puro-GFP2 Other Gift from the Watson/Patrick lab; Replication incompetent lentivirus
Strain, strain background (Lentivirus) LLV-Lenti-CMV-Hygro-DEST (w117-1)-Bloc1s1-WT This paper The de Figueiredo lab; see Methods and methods
Strain, strain background (Lentivirus) LLV-Lenti-CMV-Hygro-DEST (w117-1)-Bloc1s1-G449T This paper The de Figueiredo lab; see Methods and methods
Strain, strain background (Escherichia coli) MAX Efficiency DH5α Competent Cells Thermo Fisher Scientific Cat# 18258012
Strain, strain background (Escherichia coli) Stbl3 Other Gift from the Watson/Patrick lab; Chemically competent cells
Strain, strain background (Escherichia coli) XL10 Gold Aligent Technologies Cat# 210,518 From QuikChange Lightning Site-Directed Mutagenesis Kit
Strain, strain background (Escherichia coli) DB3.1 Campeau et al., 2009 Addgene plasmid #17,454 Containing pLenti CMV Hygro Dest (w117-1)
Genetic reagent (Mus musculus) Lyz2-Ern1 wt/wt Iwawaki et al., 2009 Gift from the Iwawaki lab
Genetic reagent (Mus musculus) Ern1mut/mut; Lyz2-Cre (Ern1 CKO) Iwawaki et al., 2009 Gift from the Iwawaki lab
Genetic reagent (Mus musculus) Xbp1 +/+ Other Gift from Dr. Laurie Glimcher
Genetic reagent (Mus musculus) Xbp1 −/− Other Gift from Dr. Laurie Glimcher
Cell line (Mus musculus) J774A.1 ATCC Cat# TIB-67;
RRID:CVCL_0358
Cells tested negative for mycoplasma; cells were maintained in cell culture media containing penicillin/streptomycin to prevent mycoplasma contamination. The cells are maintained in the Fitch lab.
Cell line (Mus musculus) Ern1+/+ MEFs Other Gift from the Kaufman Lab; cells tested negative for mycoplasma; cells were maintained in cell culture media containing penicillin/streptomycin to prevent mycoplasma contamination.
Cell line (Mus musculus) Ern1−/− MEFs Other Gift from the Kaufman Lab; cells tested negative for mycoplasma; cells were maintained in cell culture media containing penicillin/streptomycin to prevent mycoplasma contamination.
Cell line (Mus musculus) RAW 264.7 ATCC Cat# TIB-71; RRID:CVCL_0493 Cells tested negative for mycoplasma; cells were maintained in cell culture media containing penicillin/streptomycin to prevent mycoplasma contamination.
Cell line (Mus musculus) MC3T3-E1 Bae et al., 2019 Gift from the Hollien lab; cells tested negative for mycoplasma; cells were maintained in cell culture media containing penicillin/streptomycin to prevent mycoplasma contamination.
Cell line (Mus musculus) L2 Other Gift from the Leibowitz lab; this cell line is different from the L2 cell line in ATCC; this cell line can only be obtained from MHV researchers; cells tested negative for mycoplasma; cells were maintained in cell culture media containing penicillin/streptomycin to prevent mycoplasma contamination.
Transfected construct (Mus musculus) Raw 264.7 cell line: Cas9 Other Gift from the Watson/Patrick lab; cells tested negative for mycoplasma; cells were maintained in cell culture media containing penicillin/streptomycin to prevent mycoplasma contamination.
Transfected construct (Mus musculus) Raw 264.7 cell line: Cas9 mBloc1s1 This paper The de Figueiredo lab; see Methods and methods; cells were maintained in cell culture media containing penicillin/streptomycin to prevent mycoplasma contamination.
Transfected construct (Mus musculus) Raw 264.7 cell line: Cas9 Control This paper The de Figueiredo lab; see Methods and methods; cells were maintained in cell culture media containing penicillin/streptomycin to prevent mycoplasma contamination.
Transfected construct (Mus musculus) Raw 264.7 cell line: wt-Bloc1s1 This paper The de Figueiredo lab; see Methods and methods; cells were maintained in cell culture media containing penicillin/streptomycin to prevent mycoplasma contamination.
Transfected construct (Mus musculus) Raw 264.7 cell line: RIDD-resistant Bloc1s1 (Rr-Bloc1s1) This paper The de Figueiredo lab; see Methods and methods; cells were maintained in cell culture media containing penicillin/streptomycin to prevent mycoplasma contamination.
Transfected construct (Mus musculus) MC3T3-E1 cell line: RFP expressing Bae et al., 2019 Gift from the Hollien lab; cells tested negative for mycoplasma; cells were maintained in cell culture media containing penicillin/streptomycin to prevent mycoplasma contamination.
Transfected construct (Mus musculus) MC3T3-E1 cell line: Blos1s-Flag (similar to Rr-Bloc1s1) Bae et al., 2019 Gift from the Hollien lab; cells tested negative for mycoplasma; cells were maintained in cell culture media containing penicillin/streptomycin to prevent mycoplasma contamination.
Biological sample (Mus musculus) Bone marrow-derived macrophages This paper The de Figueiredo lab; see Methods and methods; cells were maintained in cell culture media containing penicillin/streptomycin to prevent mycoplasma contamination.
Antibody IRE1α antibody Novus Biologicals; Thermo Fisher Scientific Cat: NB100-2324; PA5-20189 WB (1:500–1000)
Antibody Phospho IRE1α antibody GeneTex Inc; Thermo Fisher Scientific; Abcam Cat #: GTX63722; PA1-16927; ab48187 WB (1:500–1000)
Antibody Rat polyclonal LAMP1 Antibody (1D4B) Santa Cruz Biotechnology Cat# sc-19992 IF (1:500)
Antibody Rabbit polyclonal MAP LC3β (H-50) Santa Cruz Biotechnology Cat# sc-28266 IF (1:300)
Antibody Goat polyclonal Cathepsin D Antibody (R-20) Santa Cruz Biotechnology Cat# sc-6487 IF (1:200)
Antibody Rabbit polyclonal Cathepsin D (H-75) Santa Cruz Biotechnology Cat# sc-10725 IF (1:200)
Antibody Goat polyclonal Calregulin (N-19) Santa Cruz Biotechnology Cat# sc-6468 IF (1:300)
Antibody Rabbit polyclonal Calregulin (H-170) Santa Cruz Biotechnology Cat# sc-11398 IF (1:300)
Antibody alpha Tubulin Rabbit Polyclonal Antibody Thermo Fisher Scientific Cat# PA5-29444 IF (1:1000)
Antibody Acetyl-α-Tubulin (Lys40) (D20G3) XP Rabbit mAb Cell signaling technology Cat# 5335 WB (1:1000)
Antibody Rabbit polyclonal GAPDH Antibody (FL-335) HRP Santa Cruz Biotechnology Cat# sc-25778 HRP WB (1:500)
Antibody Goat polyclonal anti-Brucella BEI Cat# DD-17 AB-G-BRU-M IF (1:400)
Antibody Brucella Rabbit Polyclonal Antibody Bioss Antibodies Cat# bs-2229R IF (1:1000)
Antibody Chicken serum anti-Blos1 This paper The de Figueiredo lab; Chicken Serum IF (1:300), WB (1:500)
Antibody Anti-SNAPIN Rabbit Polyclonal Antibody Proteintech Cat# 10055-1-AP WB (1:700)
Antibody Anti-PLDN Rabbit Polyclonal Antibody Proteintech Cat# 10891-2-AP WB (1:800)
Antibody Anti-KIF1B Rabbit Polyclonal Antibody Proteintech Cat# 15263-1-AP IF (1:200)
Antibody Anti-KIF5B Rabbit Polyclonal Antibody Proteintech Cat# 21632-1-AP IF (1:200)
Antibody Rabbit Polyclonal IHCPlus ARL8B Antibody aa72-121 Polyclonal IHC, WB LS-B5831 LifeSpan Biosciences Cat# LS-B5831-100 5 µg/ml dilution
Antibody Rabbit polyclonal C17orf59 Antibody (aa186-215) LS-C167955 LifeSpan Biosciences Cat# LS-C167955-400 WB (1:1000)
Antibody Rabbit polyclonal mTOR Antibody Cell Signaling Techology Cat# 2972 S IF (1:300)
Antibody Rabbit polyclonal KxDL motif containing 1 Antibody Novus Biologicals Cat# NBP1-82055 WB (1:500)
Antibody Rabbit polyclonal BLOS2 Polyclonal Antibody Thermo Fisher Scientific Cat# PA525452 WB (1:1000)
Antibody Alexa Fluor 488 Donkey anti-Rat IgG (H + L) Thermo Fisher Scientific Cat# A21208 IF (1:300)
Antibody Alexa Fluor 488 Donkey anti-Rabbit IgG (H + L) Thermo Fisher Scientific Cat# A21206 IF (1:500)
Antibody Alexa Fluor 488 Chicken anti-Goat IgG (H + L) Thermo Fisher Scientific Cat# A21467 IF (1:500)
Antibody Alexa Fluor 488 Chicken anti-Rabbit IgG (H + L) Thermo Fisher Scientific Cat# A21441 IF (1:300)
Antibody Alexa Fluor 594 Donkey anti-Goat IgG (H + L) Thermo Fisher Scientific Cat# A11058 IF (1:500)
Antibody Donkey anti-Rabbit IgG (H + L) ReadyProbes Secondary Antibody, Alexa Fluor 594 Thermo Fisher Scientific Cat# R37119 1 drop
Antibody Donkey Anti-Chicken IgY Antibody (Alexa Fluor 594) Jackson ImmunoResearch Cat# 703-585-155 IF (1:600)
Antibody Donkey anti Rat IgG (H + L) Secondary Antibody, Alexa Fluor 594, Invitrogen Thermo Fisher Scientific Cat# A21209 IF (1:300)
Antibody Alexa Fluor 594 Goat anti-Rabbit IgG (H + L) Thermo Fisher Scientific Cat# A11012 IF (1:300)
Antibody Alexa Fluor 647 Donkey anti-Goat IgG (H + L) Thermo Fisher Scientific Cat# A21447 IF (1:500)
Antibody Donkey anti Chicken IgY Secondary Antibody, HRP, Invitrogen Thermo Fisher Scientific Cat# SA172004 WB (1:15000)
Antibody Goat anti-Rabbit IgG HRP Santa Cruz Biotechnology Cat# sc-2004 WB (1:2000)
Recombinant DNA reagent (plasmids) See Supplementary file 1 Other and this paper See Supplementary file 1 Recombinant plasmids constructed in this work
Sequence-based reagent (oligonucleotides) See Supplementary file 2 This paper The de Figueiredo lab; See Supplementary file 2
Peptide, recombinant protein Blos1 N-terminal biotinylated Genscript Cat# SC1848 Peptide synthesized by Genscript, used to make the Chicken Serum Blos1 antibody
Commercial assay or kit Phagocytosis Assay Kit (IgG FITC) Cayman Chemical Cat# 500,290
Commercial assay or kit QuikChange Lightning Site-Directed Mutagenesis Kit Aligent Technologies Cat# 210,518
Commercial assay or kit Thermo Scientific Pierce Co Immunoprecipitation Kit Thermo Fisher Scientific Cat# 26,149
Commercial assay or kit iScript Select cDNA Synthesis Kit Bio-Rad Cat# 1708896
Commercial assay or kit Applied Biosystems High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Scientific Cat# 4368814
Commercial assay or kit RNeasy Plus Mini Kit Qiagen Cat# 74,134
Commercial assay or kit TOPO TA Cloning Kit for Subcloning, without competent cells Thermo Fisher Scientific Cat# 451,641
Commercial assay or kit Gateway LR Clonase II Enzyme mix Thermo Fisher Scientific Cat# 11791020
Chemical compound, drug 4µ8c Sigma-Aldrich Cat# SML0949 50 μM dose
Chemical compound, drug Tunicamycin from Streptomyces Sigma-Aldrich Cat# T7765 5 μg/ml dose
Chemical compound, drug SsoAdvanced Universal SYBR Green Supermix BioRad Cat# 1725274
Chemical compound, drug SuperBlock T20 (TBS) Blocking Buffer Thermo Fisher Scientific Cat# 37,536
Chemical compound, drug Western Blot Strip-It Buffer Advansta Cat# R-03722-D50
Chemical compound, drug Tween 20 Fisher scientific Cat# BP337-500
Chemical compound, drug 4% wt/vol Formaldehyde made from paraformaldehyde powder Sigma-Aldrich Cat# 158,127
Chemical compound, drug Prestained Protein Ladder – Extra broad molecular weight (5–245 kDa) Abcam Cat# ab116029
Chemical compound, drug SuperSignal West Femto Maximum Sensitivity Substrate Thermo Fisher Scientific Cat# 34,095
Chemical compound, drug RIPA Buffer Sigma-Aldrich Cat# RO278
Chemical compound, drug Phosphatase inhibitor cocktail 2 Sigma-Aldrich Cat# P5726
Chemical compound, drug Phosphatase inhibitor cocktail 3 Sigma-Aldrich Cat# P0044
Chemical compound, drug TaqMan Universal PCR Master Mix Thermo Fisher Scientific Cat# 1804045
Chemical compound, drug Puromycin Invivogen Cat# ant-pr-1
Chemical compound, drug Blasticidin Invivogen Cat# ant-bl-1
Chemical compound, drug Hygromycin Invivogen Cat# ant-hg-1
Chemical compound, drug Gram Crystal Violet Becton Dickinson Cat# 212,525
Chemical compound, drug ProLong Glass Antifade Mountant with NucBlue Stain Thermo Fisher Scientific Cat# P36981
Software, algorithm ImageJ https://imagej.nih.gov/ij/ RRID:SCR_003070 Version 1.52t (64 bit)
Software, algorithm Fiji https://imagej.net/Fiji RRID:SCR_002285 Version 2.3.0/1.53f51 (64 bit)
Software, algorithm Coloc2 https://imagej.net/Coloc_2
Software, algorithm GraphPad Prism version 8.2.0 for Windows GraphPad Software RRID:SCR_002798 https://www.graphpad.com/scientific-software/prism/
Software, algorithm Gen5 BioTek Version 3.05
Software, algorithm ICE analysis Synthego, (Synthego Performance Analysis, 2019)
Software, algorithm RNAfold WebServer University of Vienna RRID:SCR_008550 http://rna.tbi.univie.ac.at/cgi-bin/RNAWebSuite/RNAfold.cgi
Software, algorithm SnapGene GSL Biotech LLC SCR_015052 https://www.snapgene.com/
Software, algorithm STAR version 2.5.2a Dobin et al., 2013 RRID:SCR_004463 STAR: ultrafast universal RNA-seq aligner; http://chagall.med.cornell.edu/RNASEQcourse/STARmanual.pdf
Software, algorithm RSEM version 1.2.29 Li and Dewey, 2011 RRID:SCR_013027 RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome; https://deweylab.github.io/RSEM/
Software, algorithm DESeq version 1.30.0 Anders and Huber, 2010 RRID:SCR_000154 Differential expression analysis for sequence count data; https://www.bioconductor.org/packages//2.10/bioc/html/DESeq.html
Software, algorithm WebGestalt-2013 Wang et al., 2013 RRID:SCR_006786 WEB-based GEne SeT AnaLysis Toolkit (WebGestalt): update 2013; http://www.webgestalt.org
Software, algorithm PANTHER version 7 Gaudet et al., 2011; Mi et al., 2010 RRID:SCR_004869 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium; http://www.pantherdb.org
Software, algorithm edgeR version 3.26.6 McCarthy et al., 2012; Robinson et al., 2010 RRID:SCR_012802 https://www.bioconductor.org/packages/release/bioc/html/edgeR.html
Software, algorithm Cytoscape Zhao et al., 2017 RRID:SCR_003032 https://cytoscape.org/
Software, algorithm Biorender Biorender RRID:SCR_018361 https://biorender.com/
Other SeaPlaqueTM Agarose Lonza Cat# 50,101 Used for plaque assays
Other Accell Mouse Kif5b siRNA SMARTpool 5 nmol Horizon Cat# E-040710-00-0005 Used for validation
Other Accell Mouse Kif1b siRNA SMARTpool 5 nmol Horizon Cat# E-040900-00-0005 Used for validation
Other Accell Mouse Arl8b siRNA SMARTpool 5 nmol Horizon Cat# E-056525-00-0005 Used for validation
Other Accell Non-targeting Control Pool 5 nmol Horizon Cat# D-001910-10-05 Used for validation
Other Accell siRNA Delivery Media Horizon Cat# B-005000-500 Used for validation
Other ON-TARGETplus Human BLOC1S1 (2647) siRNA - SMARTpool, 5 nmol Dharmacon L-012580-01-0005 Used for validation
Other 8–16% Mini-PROTEAN TGX Precast Protein Gels, 10-well, 30 µl BioRad 4561103
Other DAPI Sigma-Aldrich Cat# D9542-10MG 1 μg/ml