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Modulation of cell-bound CEP activity by Ca2+ ions. Plasmid-free strain L. lactis MG 1363 into which the wild-type plasmid or a hybrid proteinase gene-containing plasmid was introduced was used. Activities associated with washed cells containing the Ca-free enzyme were determined at 25°C as a function of the Ca2+ concentration. Symbols: ▵, wild-type SK11 CEP (ABCD); □, hybrid CEP Abcd; ○, hybrid CEP AbCd; ●, hybrid CEP ABCd; ◬, hybrid CEP ABcD; ■, hybrid CEP AbCD.
