FIG. 6.

(a) Analytical reversed-phase HPLC of α_s1-CN(f1-23) (line a) and of the products of degradation of α_s1-CN(f1-23) by the cell-bound Ca-free CEP of strain SK11 at pH 6.5 in the absence of Ca²⁺ (120 min, 25°C) (line b) or in the presence of 0.1 mM Ca²⁺ (30 min, 25°C) (line c). (b) Analytical reversed-phase HPLC of the products of α_s1-CN(f1-23) degradation (25°C, pH 6.5) by the cell-bound Ca-free CEP of strain SK11 in the absence of Ca²⁺ (line a), at 10 mM Ca²⁺ (line b), or at 10 mM Mn²⁺ (line c).