Figure 1. MLL1i Promotes Expression of Early Cleavage Stage Gene Signatures.
(A) Left, genome-wide distribution of MLL1 relative to transcription start site (TSS). Right, G.R.E.A.T. pathway enrichment for MLL1 bound genes using —log (binomial p).
(B) Differential gene expression of MLL1i versus mock (x axis) or Mll1KO (y axis) versus Mll1f/f ESCs. Genes with FDR % 0.05 and |FC| R 1.5 were selected. The results were from biological duplicates.
(C) Genes that were up- or downregulated by MLL1i and Mll1KO were evaluated for expression dynamics in early embryonic development (Wu et al., 2016). Red, higher expression in pre-blastocyst embryos; blue, higher expression in ESCs.
(D) Heatmap (log2 fold change) for gene expression changes. Right, gene expression change induced by treatment as indicated on top. Input were genes that were upregulated (67.7%) or downregulated (56.8%) by MLL1i and Mll1KO in (C). Heatmap was generated using the defined range from —1 to 1. Left, heatmap (Z score) for the same set of genes on the right at different developmental stages indicated on top. The in vivo gene expression dataset was from Wu et al., (2016), Database: GEO: GSE66582.
(E) Heatmap for expression of selective early cleavage stage markers, indicated on the left, in mock, 2i, Mll1KO, or MLL1i cells.
See also Figures S1 and S2 and Tables S1 and S2.