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Amplification plot of allele-specific real-time PCR showing PIK3CA mutations in exon 9 and exon 20 and the HEX (green, wild-type PIK3CA allele) and FAM (blue, mutated PIK3CA allele) signals. (A) A missense mutation in sample No. PS212 at position 1633, G>A (E545K) in exon 9. (B) A missense mutation in sample No. PS243 at position 3140, A>G (H1047R) in exon 20. (C) A silent mutation in sample No. PS251/1, C>T (T1025T) in exon 20. PCR, polymerase chain reaction.
