Table 10.

Effects of subclinical necrotic enteritis challenge, diet type and supplemental enzymes on ileal volatile fatty acids (μmol/g) in broilers at 21 d of age.^1,2

Diet type	Enzyme	Formic	Acetic	Propionic	Butyric	Total VFA3	Succinic	Lactic
Two-way interaction
Wheat	Control	0.5ab	4.7	0.15	0.15	5.6	0.50	41.7
	XYN10	0.3b	4.8	0.27	0.03	5.4	0.60	42.9
	XYN11	1.0ab	5.4	0.15	0.12	6.6	0.48	34.5
	MAN	0.3b	5.0	0.15	0.37	5.8	0.62	55.2
Maize	Control	0.3b	4.5	0.22	0.01	5.1	0.79	69.7
	XYN10	1.2a	4.7	0.24	0.12	6.3	0.37	33.2
	XYN11	0.8ab	4.3	0.16	0.06	5.3	0.44	35.1
	MAN	0.7ab	4.9	0.17	0.01	5.8	0.62	55.1
Main effects
Challenge	No	0.6	4.6	0.12b	0.13	5.5	0.43b	42.4
	Yes	0.7	5.0	0.26a	0.09	6.0	0.68a	49.7
Diet type	Wheat	0.5	5.0	0.18	0.18	5.9	0.55	43.8
	Maize	0.7	4.6	0.20	0.05	5.6	0.55	48.3
Enzyme	Control	0.4	4.6	0.19	0.08	5.3	0.64	55.7b
	XYN10	0.8	4.8	0.26	0.08	5.8	0.49	38.0a
	XYN11	0.9	4.9	0.15	0.10	6.0	0.46	35.3a
	MAN	0.5	5.0	0.16	0.19	5.8	0.62	55.1b
SEM4		0.062	0.19	0.021	0.047	0.05	0.221	3.34
P-value
Challenge		0.823	0.377	<0.001	0.652	0.301	0.013	0.254
Diet type		0.071	0.358	0.556	0.188	0.569	0.973	0.489
Enzyme		0.020	0.926	0.274	0.812	0.710	0.441	0.041
Challenge × diet type		0.378	0.194	0.590	0.150	0.245	0.181	0.053
Challenge × enzyme		0.381	0.875	0.200	0.494	0.802	0.601	0.271
Diet type × enzyme		0.003	0.755	0.906	0.424	0.380	0.274	0.176
Challenge × diet type × enzyme		0.957	0.664	0.994	0.595	0.586	0.625	0.749

^a-b Within a column, means with no common superscripts differ significantly (P < 0.05).

¹Means are based on 6 replicates per treatment with 3 birds per replicate.

²Control = no enzyme; XYN10 = xylanase from family 10; XYN11 = xylanase from family 11; MAN = β-mannanase.

³Total volatile fatty acids = acetic acid + propionic acid + butyric acid + valeric acid + branched short-chain fatty acids.

⁴SEM = standard error of the mean.