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. 2022 Mar 17;10:54–67. doi: 10.1016/j.aninu.2022.01.009

Table 9.

Effects of subclinical necrotic enteritis challenge, diet type and supplemental enzymes on ileal volatile fatty acids (μmol/g) in broilers at 16 d of age.1,2

Challenge Diet type Enzyme Formic Acetic Propionic Butyric Total VFA3 Succinic Lactic
Two-way interactions
No Control 0.13 2.7 0.24 0.11 3.1 0.09 13.7c
XYN10 0.05 2.4 0.23 0.00 2.7 0.15 14.4c
XYN11 0.00 2.7 0.15 0.00 2.9 0.17 20.4c
MAN 0.06 2.6 0.19 0.14 3.0 0.10 14.0c
Yes Control 0.06 3.0 0.16 0.74 4.0 0.36 34.0b
XYN10 0.13 2.6 0.21 0.14 3.1 0.35 33.4b
XYN11 0.05 3.0 0.19 0.36 3.6 0.46 33.1b
MAN 0.09 2.3 0.60 0.42 3.6 0.66 44.4a
Wheat Control 0.16a 3.4 0.22 0.92 4.7 0.18 18.2c
XYN10 0.05b 2.3 0.19 0.04 2.6 0.32 29.8abc
XYN11 0.02b 2.9 0.17 0.18 3.3 0.34 35.3a
MAN 0.03b 2.4 0.16 0.19 2.8 0.24 25.2abc
Maize Control 0.03b 2.3 0.19 0.00 2.5 0.28 29.8abc
XYN10 0.13a 2.7 0.25 0.11 3.2 0.18 18.3bc
XYN11 0.03b 2.8 0.17 0.20 3.2 0.31 19.4bc
MAN 0.11a 2.5 0.62 0.37 3.8 0.52 32.1ab
Main effects
Challenge No 0.06 2.6 0.20 0.07 2.9 0.12a 15.7
Yes 0.08 2.8 0.29 0.41 3.6 0.46b 36.4
Diet type Wheat 0.07 2.8 0.19 0.31 3.3 0.26 26.9
Maize 0.08 2.6 0.31 0.17 3.2 0.32 25.2
Enzyme Control 0.10 2.8 0.21 0.43 3.6 0.22 23.7
XYN10 0.09 2.5 0.22 0.07 2.9 0.25 23.7
XYN11 0.03 2.8 0.17 0.18 3.2 0.31 27.2
MAN 0.07 2.5 0.39 0.28 3.3 0.38 29.6
SEM4 0.016 0.12 0.056 0.089 0.22 0.046 1.54
P-value
Challenge 0.607 0.519 0.442 0.050 0.135 <0.001 <0.001
Diet type 0.841 0.519 0.282 0.409 0.771 0.501 0.307
Enzyme 0.361 0.578 0.501 0.524 0.751 0.541 0.033
Challenge × diet type 0.392 0.292 0.361 0.934 0.926 0.818 0.068
Challenge × enzyme 0.334 0.809 0.399 0.800 0.978 0.990 0.001
Diet type × enzyme 0.039 0.226 0.363 0.143 0.128 0.074 <0.001
Challenge × diet type × enzyme 0.369 0.718 0.374 0.181 0.242 0.119 0.307

a-c Within a column, means with no common superscripts differ significantly (P < 0.05).

1

Means are based on 6 replicates per treatment with 4 birds per replicate.

2

Control = no enzyme; XYN10 = xylanase from family 10; XYN11 = xylanase from family 11; MAN = β-mannanase.

3

Total volatile fatty acids = acetic acid + propionic acid + butyric acid + valeric acid + branched short-chain fatty acids.

4

SEM = standard error of the mean.