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. 2022 May 9;19(5):586–593. doi: 10.1038/s41592-022-01460-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, iNTA scatter plots of synthetically produced liposomes. Color bar denotes point density. The gray dashed contours correspond to different refractive indices of the inner part of the liposomes, starting at 1.334 (water) and increasing in steps of 0.02 from 1.34 for n_sh = 1.48 and t_sh = 5.7 nm. Inset: cartoon of a vesicle. b, Isosurfaces of constant particle size and iSCAT contrast for the points marked in a over a range of values for n_in, n_sh and t_sh. c, iNTA scatter plots of EVs from Leishmania parasites. The green line indicates the best fit value for n_in, while the solid (dashed) olive lines indicate the 25th and 75th (10th and 90th) percentiles of the extracted n_in. d, Same as b but for the points marked in c. e, iNTA scatter plots of EVs from urine of a healthy human donor. The red line indicates the best fit value for n_in, and the solid (dashed) maroon lines indicate the 25th and 75th (10th and 90th) percentiles of the extracted n_in. f, Same as b but for the points marked in e. Each plot shows the outcome of one iNTA measurement performed on one sample. The results from more samples are shown in Supplementary Fig. 18.