TABLE 1.
Active ingredients of selected disinfecting products and appropriate neutralizers
| Product | Active ingredient | Label contact time (min)a |
pH | Neutralizer (concentration) | Cytotoxicity/CPE after neutralization | |
|---|---|---|---|---|---|---|
| FCV | C. difficile | |||||
|
| ||||||
| A | 0.5% hydrogen peroxide | 1 | NA | 2.64 | catalase (1300 U l−1) | −b |
| B | 0.88% hydrogen peroxide | 10 | NA | 2.85 | catalase (1300 U l−1) | − |
| C | 1.4% hydrogen peroxide | 1 | NA | 2.38 | catalase (1300 U l−1) | − |
| D | 3.13% hydrogen peroxide/0.099% octanoic acid/0.05% peracetic acid | 4 | 10 | 2.95 | catalase (1300 U l−1) | − |
| E | 5% hydrogen peroxide/0.005% silver | 10 | NA | 3.07 | catalase (1300 U l−1) | − |
| F | 4.85% citric acid/0.003% silver | 10 | NA | 1.79 | FBS (5%) | − |
| G | 0.2% chlorine dioxide/0.125% alkyl dimethyl benzyl ammonium chloride/0.125% alkyl dimethyl ethylbenzyl ammonium chloride | 5 | NA | 8.68 | FBS (5%) + sodium thiosulphate (0.1%) | − |
| H | 15% isopropanol/7.5% ethanol/0.76% didecyldimethylammonium chloride | 1 | NA | 12.17 | FBS (5%) + sodium thiosulphate (0.1%) | − |
| I | 29.4% ethanol | 0.5 | NA | 13.07 | FBS (5%) | +c |
a
Recommended contact time against FCV listed on product labels; NA represents ‘not available’.
b
No cytotoxicity/CPE to both cell lines after neutralization, that is, <1 log reduction of viruses or spores in neutralization effectiveness treatments.
c
Cytotoxicity of samples was finally neutralized by washing with centrifugal filters.