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. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: Small. 2022 Mar 21;18(20):e2107795. doi: 10.1002/smll.202107795

Figure 5: Potential application of fully automated NFP-E system in cell line generation workflow.

Figure 5:

The automated NFP-E system can be used to generate isogenic cell lines with targeted mutations. Arrays of single or few cells in micro-confinements can be transfected with the Cas9/sgRNA machinery using the NFP-E (1). Single cell clones are then generated by antibiotic selection or by isolating colonies originating from single cells (2). The harvested clones may be expanded and validated to obtain isogenic cell lines (3) and then used for drug screening and disease modeling studies (4)