(A) Diagram of the experimental strategy to examine astrocyte and microglia activation within the spinal cord along with Tdp-43 localization. (B) Diagram of cell types analyzed in the spinal cords. (C) Stmn2 F0 genotype corresponding to the spinal cord analysis. Hemi-sections of the lumbar spinal cord from Stmn2 F0 mice and Rosa26 F0 controls were stained for (D) GFAP, (E) Iba1, and (F) Tdp-43 and quantified in motor neurons (F). Graphs of cell counts (G), ns, p>0.05 (n=3 animals/genotype, n=5 sections/animal, for Tdp43 localization analysis: n=100 Chat+ neurons counted/animal)