FIGURE 7.

Phox2b identifies glutamatergic and catecholaminergic populations ventral and medial to the PB. DAB in situ hybridization revealed Phox2b mRNA, shown at three rostral‐to‐caudal levels (a–c). Approximate level caudal to bregma is shown at the bottom‐right of each panel (in mm). (d–f) Immunofluorescence labeling for Phox2b (green) and tyrosine hydroxylase (TH, magenta) identify LC neurons. (g–i) FISH identified colocalization between Phox2b mRNA (ice‐blue) and the glutamatergic marker Slc17a6 mRNA (h, red) ventral to a mid‐level of the PB (bregma −5.1 mm). Ubc mRNA (green) is shown for neuroanatomical background. (j–l) Blow‐ups of the highlighted region in (g–i) show the ubiquitous colocalization of Slc17a6 mRNA in Phox2b‐expressing neurons in the supratrigeminal region. All scale bars are 200 μm and apply to related panels. Abbreviation: V, motor trigeminal nucleus