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. 2022 May 19;13(5):477. doi: 10.1038/s41419-022-04934-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Schematic diagram of binding sites between circ-Pank1 and the α-syn mRNA 3’UTR and miR-7a-5p. B The regulatory effect of circ-Pank1 on the expression of miR-7a-5p was verified by dual-luciferase reporter gene detection. The psi-circ-Pank-WT/Mut plasmid and miR-7a-5p mimic were cotransfected into HEK293T cells, and the luciferase activity was measured after 24 h (n = 3). C qRT–PCR was used to detect the expression of miR-7a-5p in the SN of PD model mice treated with rotenone (n = 4). D After si-circ-Pank1 and the corresponding control were transferred to the MN9D cell model treated with rotenone, the expression changes of miR-7a-5p were detected by qRT–PCR (n = 3). E The regulatory effect of miR-7a-5p on α-syn expression was verified by the dual-luciferase reporter gene detection. The psi-α-syn WT/Mut plasmid and miR-7a-5p mimic were co-transfected into HEK293T cells. After 24 h, the luciferase activity was measured. F–H After the transfection of miR-7a-5p mimics in MN9D cells (n = 3), Western blot analysis of α-syn protein levels F, G qRT–PCR detection of α-syn mRNA expression H. Data are presented as mean ± SD. *P < 0.05, **P < 0.01.