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. 2022 May 19;5:483. doi: 10.1038/s42003-022-03427-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, b Immunofluorescence of phosphorylated STAT1 (PY-STAT1) in HeLa cells transfected with AcGFP or AcGFP-ORF6 following interferon-β (IFN-β) a or IFN-γ b stimulation. GFP (green) and PY-STAT1 (red) were identified using specific antibodies. DAPI staining (blue) was used for DNA staining. Scale bars: 30 μm. c The graph represents the relative fluorescence values of PY-STAT1 in the nucleus compared to those of the whole cells (shown in b). Signal intensities of total 100 different nuclei from two independent experiments. ***P < 0.001, two-tailed Student’s t-test. error bars represent SD. d qRT-PCR analysis of IP-10 mRNA in AcGFP or AcGFP-ORF6-transfected HeLa cells at the described time points (n = 4 each). *P < 0.05, ***P < 0.001, two-way ANOVA. error bars represent SD. e, f Relative luciferase values of GAS-Luc e or ISRE-Luc f in AcGFP- or AcGFP-ORF6-transfected Huh7 cells following IFN-γ stimulation (n = 3 each). ***P < 0.001, one-way ANOVA. error bars represent SD. g, h Relative luciferase values of GAS-Luc g or ISRE-Luc h in VeroE6/TMPRSS2 cells infected with SARS-CoV-2 (NIID strain) following IFN-γ stimulation (n = 3 each). *P < 0.05, ***P < 0.001, one-way ANOVA. error bars represent SD.