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. 2022 May 19;11(1):26. doi: 10.1038/s41389-022-00401-x

Fig. 3. p53 elevation promotes mitochondrial elongation accompanied by attenuated invasive cell migration.

Fig. 3

A Immunoblot of p53 in siCtrl and sip53 A549 cells with and without 20 µM SA treatment for 24 h. GAPDH was used as a loading control. Representative images (B) and quantification (C) of mitochondrial morphology in siCtrl (n = 223), siCtrl+SA (n = 211), sip53 (n = 205), and sip53+SA (n = 218) A549 cells. Boxed regions in (B) are shown enlarged in the bottom panels. Scale bar: 20 µm. Migration distance (D) and representative trajectories (E) of siCtrl (n = 29), siCtrl+SA (n = 17), sip53 (n = 13), and sip53+SA (n = 15) A549 cells. Transwell assays for (F) siCtrl, siCtrl+SA, sip53, and sip53+SA A549 cells or (G) control (Ctrl) and p53-overexpressing (p53) H1299 cells. Scale bar: 100 µm. Error bars represent mean ± SD. Data were analyzed by one-way ANOVA with Tukey’s multiple comparisons test. ****p < 0.0001.