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. 2022 May 19;13:2762. doi: 10.1038/s41467-022-30444-y

Fig. 4. PTEN deficiency enhances platelet aggregation and cytokines secretion through activation of PDK1/mTORC2-AKT-SNAP23 pathway.

Fig. 4

The pLNs of 6-month-old mice were subjected for analysis (a–g). a Platelet counts (left) and megakaryocyte (right) ratio in peripheral blood or bone marrow of Ptenfl/fl (platelet, n = 7; megakaryocyte, n = 4) and Ptenfl/flPf4-Cre mice (platelet, n = 8; megakaryocyte, n = 4). b Representative flow cytometry analysis of the surface P-selectin on Ptenfl/fl and Ptenfl/flPf4-Cre platelets stimulated with 0.1 U/ml Thrombin. c Ptenfl/fl and Ptenfl/flPf4-Cre platelets aggregation in response to 0.1 U/ml Thrombin, 1 µg/ml Collagen and 10 µM ADP, respectively. d Relative levels of cytokines released from platelets of Ptenfl/fl and Ptenfl/flPf4-Cre mice stimulated with 0.1 U/ml Thrombin (n = 4). Data are presented as fold changes relative to wild-type platelets. e The pathway signatures in response to IL-6, IL-17, and IFN-γ were significantly overrepresented in CD4+ T cells isolated from pLNs from Ptenfl/flPf4-Cre mice. f Western blotting of indicated proteins in wild-type and Pten-deficient platelets in response to 0.1 U/ml Thrombin with the presence of DMSO or indicated inhibitors. g Representative flow cytometry analysis of the surface P-selectin on Ptenfl/fl platelets stimulated with 0.1 U/ml Thrombin in the presence of DMSO or indicated inhibitors. Results are representative of three independent experiments. The data shown in (a, d) are presented as mean ± s.e.m. (two-tailed t test). Source data are provided as a Source Data file.