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. 2022 May 19;13:2786. doi: 10.1038/s41467-022-30437-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — 8–10 week old control and Cul5^fl/flCD4-Cre mice were treated with HDM or PBS. Hematoxylin and Eosin (H&E) and Periodic Acid-Schiff (PAS) stain of lungs. Representative image (a) of H&E and PAS stain (combined data shown in panels b and c). Bar graph (b) showing the number of nuclei enumerated by Aperio ImageScope software in 100 mm² area. n = 6 control, n = 7 Cul5^fl/flCD4-Cre, examined in two independent experiments. Bar graph showing PAS score (c) in lungs. n = 7 control and Cul5^fl/flCD4-Cre mice, examined in two independent experiments. Total number of eosinophils (d), T cells (e), and CD4⁺ T cells (f) in BALF. n = 4 control, n = 5 Cul5^fl/flCD4-Cre examined in 1 experiment. Frequencies (g) and numbers (h) of eosinophils in the lungs. n = 7 for Cul5^fl/flCD4-Cre HDM animals; n = 8 for all other groups examined in two independent experiments. i Percentages of IL-5⁺ cells in lungs. For PBS group, n = 8 control and Cul5^fl/flCD4-Cre mice, examined over two independent experiments. For HDM group n = 10 control, n = 12 Cul5^fl/flCD4-Cre mice, examined in three independent experiments. j IL-5⁺ cell numbers in lungs. n = 12 control and Cul5^fl/flCD4-Cre mice examined in three independent experiments. Frequencies (k) and numbers (l) of IL-13⁺ cells. For PBS group, n = 4 control, n = 5 Cul5^fl/flCD4-Cre, examined in one experiment. For HDM group, n = 7 control, n = 11 Cul5^fl/flCD4-Cre, examined in two independent experiments. Frequencies (m) and numbers (n) of IL-9⁺ cells. For PBS group n = 8 control and Cul5^fl/flCD4-Cre mice. For HDM group n = 7 control and Cul5^fl/flCD4-Cre mice, examined in two independent experiments. Frequencies (o) and numbers (p) of mast cells (FcεRIα^hic-Kit^hi). For PBS group n = 4 control, n = 5 Cul5^fl/flCD4-Cre examined in one experiment. For HDM group n = 7 control, n = 11 Cul5^fl/flCD4-Cre, examined in two independent experiments. q Airways resistance measured by forced inspiration using Buxco® FinePointe system in mice administered with increasing doses of methacholine. For PBS group n = 8 control and Cul5^fl/flCD4-Cre mice; for HDM group n = 7 control and Cul5^fl/flCD4-Cre mice, examined in two independent experiments. Data is presented as Mean ± SEM in panels b–q. p-value is calculated by unpaired two-tailed t-test. Source data are provided as a Source Data file.