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. 2022 May 19;13:2782. doi: 10.1038/s41467-022-30066-4

Fig. 2. Large granules in old flies are dynamic RNP assemblies.

Fig. 2

a–c Cell bodies of MB γ neurons imaged from 37–38 day-old (aged) brains. Me31B-GFP-expressing brains were stained with anti-Ubiquitin (a, magenta in a”) antibodies, anti-p62 (b, magenta in b”) antibodies, or profilin smFISH probes (c, magenta in c”). Me31B-GFP distribution is shown in white in a’-c’ and in green in a”-c”. Arrowheads in c” point to large granules containing profilin mRNA. Insets show magnifications of the boxed region in c,c’. Two independent biological replicates were performed. Scale bar: 5 μm. d Average FRAP curves obtained after photobleaching of GFP-Imp-positive (green) or Me31B-GFP-positive (red-orange) granules from 1–2 day- (young) or 38 day- (aged) old brain explants. A minimum of 90 granules from at least 9 brains were analyzed per condition. Error bars (fill areas) represent s.e.m. Source data are provided as a Source Data file.