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. 2022 May 19;5:479. doi: 10.1038/s42003-022-03432-7

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© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Schematic diagram of breeding strategy for the generation of IL-33 knockout mice. b Eyes from IL-33^+/+ and IL-33^−/− pups were enucleated, retinas isolated, and extracts were analyzed for IL-33 levels by Western blotting and normalized to β-tubulin. c–e Everything is same as in b, except that retina from IL-33^+/+ and IL-33^−/− mice pups were stained with isolectin B4, and flat mounts were prepared and examined for endothelial tip cell formation (c) retinal neovascularization (d), and avascular area (e). The middle column in d shows the higher magnification of the area selected. Neovascularization is highlighted in red in the third column of d. The bar graphs represent quantitative analysis of percentage of neovascularization and avascular area, expressed as Mean ± SD. *P < 0.05 vs control siRNA. Scale bar represents 20 μm in c upper row, 50 μm in c lower row, 500 μm in d and e.