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. 2022 May 19;12:8456. doi: 10.1038/s41598-022-11819-z

Figure 6.

Figure 6

Mucin-related glycosyl hydrolase profiles in the Firmicutes phlyum. (AC) Heat map of the Firmicutes genomes that have at least one gene copy of mucin-associated GH 33, 16, 29, 95, 20, 2, 35, 42, 98, 101, 129, 89, 85, and 84. Heat map showing the gene copy number of mucin-associated GHs in the strains of (D) Abiotrophia defective, Blautia coccoides, B. hansenii, B. obeum, B. producta, Blautia spp., Enterococcus casseliflavus, E. durans, E. gallinarum, and Enterococcus spp., (E) Paenibacillus, specifically Paenibacillus spp., P barcinonensis and P. lautus, (D) Ruminococcus, including Ruminococcus spp. R. gnavus and R. torques, (F) Streptococcus, including S. australis (Sa), S. intermedius (Si), S. mitis (Sm), Streptococcus spp. and Viridibacillus spp. (G) Clostridium, including C. butyricum (Cb), C. sporogenes (Cs), and Clostridium spp. (H,I) Growth analysis of Clostridium butyricum CB, Clostridium symbiosum ATCC 14940, Clostridium inoculum ATCC 14501, Clostridium clostridiforme ATCC 25532, and Clostridium sporogenes DSMZ 795 (H), as well as Lactobacillus gasseri ATCC 33323, L. johnsonii ATCC 33200, L. brevis ATCC 27305, L. acidophilus ATCC 4796 (I) in a chemically defined media ZMB1 lacking glucose (media control), with glucose (positive control), or lacking glucose and supplemented with 1 mg/mL porcine intestinal MUC2. Growth was measured by examining the optical density at 600 nm (OD600nm) after overnight incubation.