Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 19;13:2785. doi: 10.1038/s41467-022-30466-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 7 — A, B TTX (1 µM; A; vehicle n = 6, TTX n = 6; P < 0.0001) and lamotrigine (LTR; 200 µM; B; vehicle n = 9, LTR n = 9; P = 0.0001) reduce Nf1^+/neo DRG neuron Col1a2 secretion by 73 and 47% relative to vehicle-treated controls. C ZD7288 (ZD; 30 µM) increases Col1a2 secretion in WT (n = 10 in both groups; P < 0.0001) and Nf1^+/1809 (n = 4 in both groups; P = 0.0103) DRG neurons. D RAS activity is increased in both Nf1^+/neo and Nf1^+/1809 DRG neurons relative to controls (n = 5 in all groups; P < 0.0001), (E) and is inhibited following TTX and LTR treatment (n = 6 in all groups; P < 0.0001). F, G IN-1 has no effect on DRG neuronal activity, as measured by (F) multi-electrode array (vehicle n = 6, IN-1 n = 3, ns not significant), or (G) calcium-imaging recordings (vehicle n = 18, IN-1 n = 18; ns, not significant). Right: representative (F) spike plots of entire multi-electrode array well recordings over 30 s, and (G) traces of neuronal activity over 3 min. H IN-1 reduces Col1a2 secretion by 77.9% in Nf1^+/neo DRG neurons. n = 6 for both groups, P = 0.0001. I IN-1 reduces proliferation by 50% in Nf1^−/− DRG–NSCs. n = 6 for both groups, P < 0.0001. J Lamotrigine treatment decreases pNF progression in vivo. Gross images and representative immunostaining of mouse pNFs demonstrate that LTR treatment reduces pNF size, partly restores neuronal histology (H&E), reduces proliferation (Ki67⁺ cells) and decreases Col1a2 production. Scale bars: gross anatomy images, 1 mm; sections, 100 µm. n = 5 for both groups. Data are represented as means ± SEM (A–C, H, I) using two-tailed paired Student’s t tests, (F, G) two-tailed unpaired t tests, or (D, E) one-way ANOVA with Dunnett’s post-test correction. P values are indicated within each panel. ns, not significant. Source data are provided as a Source Data file.