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Regulatory T cell (Treg) staining of CD4+cells isolated from MLNs. (A) Representative density plot of Treg gating strategy. (B) Frequencies of CD4+CD25+Foxp3+ cells in MLNs of WT, Winnie, and Winnie-TNF-KO mice treated with anakinra or vehicle (N = 9 for each group). ∗P < .05. FSC, forward scatter; SSC, side scatter.
