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. 2022 Apr 20;298(6):101954. doi: 10.1016/j.jbc.2022.101954

Figure 3.

Figure 3

Structural analysis of the PfRACK1 protein binding to Plasmodium falciparum 40S ribosome subunit. All structures displayed are Plasmodium falciparum proteins/RNA. A, the structure of the P. falciparum 80S ribosome (RCSB PDB ID: 3jbo) was structurally aligned with the human 80S ribosome (RCSB PDB ID: 3jag). The de novo model of PfRACK1 generated by SWISS-MODEL was structurally aligned with that of the human 80S bound RACK1. The view shown is that which has been previously published arguing for the importance of the regions noted in RACK1 binding to the ribosome. Solid circle: region vital for HsRACK1:Hs80S binding. Dotted circle: Loop region that varies highly from species to species, and while not required, may influence binding. B, the same view as above, however, ribosomal-binding proteins with potential interactions are shown. C, a 180-degree flipped view to show additional RPS16 (uS9) and RACK1 interactions. Note: Region in blue is that when exchanged between human and P. falciparum enabled binding of parasite RACK1 to the human 40S ribosome. HsRACK1, Homo sapiens RACK1; PfRACK1, Plasmodium falciparum RACK1; RACK1, receptor for activated C-kinase 1.