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. 2022 Apr 20;298(6):101954. doi: 10.1016/j.jbc.2022.101954

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Airyscan confocal fluorescence imaging microscopy of mNeonGreen-tagged PfRACK1 expressed in Plasmodium falciparum.A, Hoechst stain (DNA), BODIPY TR ceramide (lipid bilayers), mNeonGreen (RACK1), transmitted-photomultiplier tube (T-PMT) as brightfield, and overlay images. Top: Dd2 parent line. Middle: Dd2 parent line expressing mNeonGreen-tagged PfRACK1 at trophozoite stage. Bottom: Dd2 parent line expressing mNeonGreen-tagged PfRACK1 at schizont stage. B, two-dimensional overlay using Imaris software analysis of Airyscan images. Left: Dd2 parent line at the schizont stage. Right: Dd2 parent line expressing mNeonGreen-tagged PfRACK1 in trophozoite and schizont stages. The size bar in the images represents 5 μM. PfRACK1, Plasmodium falciparum RACK1; RACK1, receptor for activated C-kinase 1.