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. 2022 Apr 20;298(6):101954. doi: 10.1016/j.jbc.2022.101954

Figure 7.

Figure 7

Ribosomal binding of RACK1 variants in mammalian HAP1 ΔRACK1 cells and Plasmodium falciparum parasites.A, Western blot of lysates and postsucrose cushion centrifugation pellets of HAP1 ΔRACK1 cells expressing N-terminally 3xFlag tagged RACK1 variants. 40S ribosomal protein 16 (uS9) blotted as control. B, Western blot of lysates and postsucrose cushion centrifugation pellets from Plasmodium falciparum parasites expressing C-terminally 3xHA-tagged RACK1 variants. C, ribbon model (above) displays ribosome-facing structure with colored region indicating N-terminal section previously shown to be important for binding in mammalian cells. Electrostatic map (below) of above ribbon model ribosome-facing surface for human and P. falciparum RACK1 proteins generated using PyMOL APBS Electrostatics plugin. Outlines indicate colored region of N-terminal section previously shown to be important for RACK1 binding in mammalian cells. RACK1, receptor for activated C-kinase 1.