Figure 11.

Decreased IL-10 expression in Lpar5-deficient splenocytes. (A) Il-10 mRNA expression was determined in WT and Lpar5^KO (KO) splenocytes. n = 3. ∗P < .05. (B) The effects on splenocytes on Cxcl10 mRNA expression in Lpar5^KO organoids were determined. N indicates no splenocytes; W indicates co-cultured with WT splenocytes; and K indicates co-cultured with Lpar5^KO splenocytes. n = 4. ∗∗P < .01; ∗∗∗P < .001. (C) Cxcl10 mRNA levels were determined in Lpar5^KO organoids with or without IL-10. n = 4. ∗∗P < .01. (D) Lpar5^KO enteroids were cultured with or without 100 ng/mL IL-10. Numbers of viable enteroids were counted. n = 6. ∗∗∗P < .001; ∗∗∗∗P < .0001. (E) Cxcl10 expression levels were determined in Lpar5^KO enteroids co-cultured with Lpar5^KO splenocytes (KO), Lpar5^KO splenocytes+IL10 (KO+IL10), WT splenocytes (WT), or WT splenocytes+α-IL10 antibody (WT+αIL10). n = 4. ∗∗∗P < .001; ∗∗∗∗P < .0001. (F) Representative confocal IF microscopic images of enteroids stained for EdU or TUNEL under the same conditions described previously. Quantification of (G) EdU⁺ or (H) TUNEL⁺ per enteroid are shown. n = 10. ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001. ns indicates not significant by 1-way analysis of variance with Tukey’s multiple comparison test.