Figure 2.

Lpar5 loss induces in crypt epithelial cell apoptosis. (A) Representative images of immunohistochemical staining of CC3 in the jejunum of Lpar5^f/f and Lpar5^f/f;RosaCre^ERT mice treated with 5× TAM. Apoptosis of IECs in the crypt compartment (blue arrows) of Lpar5^KO mice is indicated. Scale bar = 100 μm. (B) Lpar5 mRNA expression in Lpar5^f/f;RosaCre^ERT mice treated with 100 mg/kg TAM was determined by quantitative RT-PCR. n = 3. ∗∗∗P < .001 compared with 0 hours. (C) Representative immunofluorescence images of apoptosis (TUNEL, red) and proliferation (EdU, green) of crypt epithelial cells in the jejunum of Lpar5^f/f and Lpar5^KO mice on each day of treatment are shown. Representative of 3 experiments. Scale bar = 50 μm. (D) Quantification of TUNEL⁺ apoptosis per crypt from the jejunum. n = 30. (E) Quantification of EdU⁺ cells per crypt. n = 40. Representative of 3 independent experiments. All data are presented as mean ± SD. ∗P < .05, ∗∗∗P < .001 compared with day 0 (D0) by 1-way analysis of variance with Tukey’s multiple comparison test. (F) Representative images of EdU and TUNEL staining in the jejunal sections of Lpar5^f/f mice treated with 100 mg/kg/d for 2 days are shown. Quantification of EdU⁺ and TUNEL⁺ cells per crypt on days 0, 2, and 4 are shown on the right. n = 90.