Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 4;14(1):129–150. doi: 10.1016/j.jcmgh.2022.03.012

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

LPA₅is necessary for the growth of enteroids. (A) Lpar5^f/f and Lpar5^f/f;RosaCre^ERT mouse enteroids were treated with 1 μM 4OHT or ethanol (Cont) on day 0 (D0). Representative images taken on day 2 (D2) are shown. Data are representative of >4 experiments. (B) The growth of enteroids in the presence of 4OHT was quantified by determining the surface area of enteroids using ImageJ 1.53k. All data are presented as mean ± SD. ∗∗∗P < .001, ∗∗∗∗P < .0001 compared with D0 by 2-way analysis of variance with Tukey’s multiple comparison test. n = 10. (C) Representative immunofluorescence images of EdU (green) and TUNEL (red) taken on D2 are shown. DAPI = blue. (D) WT enteroids were treated with TC LPA5 4 (5 μM), an antagonist for LPA₅ for 3 days. Representative images from 3 experiments are shown.