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. 2022 May 19;12:8375. doi: 10.1038/s41598-022-12128-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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OCT B-scan image of CAM showing an arteriole (red)/venule (blue) pair irradiated at the same fluence rate. (A) Initial OCT B-scan before laser irradiation. (B) Coagulum formation observed in both arterioles and venules. (C) In the arteriole, the coagulum holds, resulting in hemostasis, while in the venule, the coagulum breaks free near the wall. (D) Blood flow is restored in the venule where the coagulum breaks from the vessel wall (Panel C). Please see supplementary Fig. S10 for enface images collected from this experiment. Scale bar 500 µm. These arteriole/venule pairs were chosen on the CAM model appropriately sized at Day 7 with direct connection to major CAM vessels.