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. 2022 May 19;13:2783. doi: 10.1038/s41467-022-30452-y

Fig. 1. Enteric neurons AVL and DVB fire action potentials with distinct kinetics.

Fig. 1

a, b Representative current-clamp recording traces from DVB and AVL. Top: current-injection stimulation steps (5 s long at 1 pA increment). Left: membrane potential dynamics under color-matched stimulation steps. Right: representative single recording traces with action potentials fired. c Statistical measurements of resting membrane potential (DVB: −49.0 ± 3.6 mV; AVL: −45.7 ± 1.1 mV), threshold (DVB: −23.4 ± 1.6 mV; AVL: −23.3 ± 0.8 mV), peak amplitude (DVB: 26.3 ± 2.6 mV; AVL: 9.8 ± 0.8 mV), afterhyperpolarization (AHP) (DVB: −38.5 ± 2.5 mV; AVL: −64.8.3 ± 1.2 mV), and half-maximum spike width (DVB: 358.8 ± 59.5 ms; AVL: 394.2 ± 40.0 ms) for DVB (n = 5) and AVL action potentials (n = 17) in wild-type animals. The error bar is SEM. d, e Representative calcium imaging traces from the cell bodies of DVB and AVL expressing GCaMP6f. Left: 300 s continuous recording traces. Right: isolated single calcium spikes from the left traces to demonstrate the difference in calcium signal decay. Source data are provided as a Source Data file.