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. 2022 May 19;13:2771. doi: 10.1038/s41467-022-30514-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Heat maps show 3 independent biological replicates at each condition, counting the most PAM-distal position as 1. The dashed line in scatters dot plots shows the mean value of all desired BE efficiencies within the displayed activity windows of each site. Single-site, using CBE-dCas12a or ABE-dCas12a with the relevant hU6-driven gRNA for single-site base editing. Detailed constructs of CBE-dCas12a (2xUGI-rAPOBEC1-dCas12a) and ABE-dCas12a (TadA-8e-dCas12a) are available in Supplementary Table 1. Protospacer and amplicon information can be found in Supplementary Table 5.