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Optimized chromatographic conditions.
| Parameter | Optimized condition |
|---|---|
| Chromatograph | HPLC (Agilent 1260) |
| Column | Eclipse Plus C18 (4.6 mm × 100 mm) |
| Flow rate | 1 mL/min |
| Detection wavelength | 218 nm |
| Mobile phase | Trifluoroacetic acid in water, acetonitrile, and methanol (70:25:5 v/v) |
| Injection volume | 20 μl |
| Column temperature | 40 °C |
