Fig. 3.

Antidepressant-like effects of Rb1 on synaptic structural changes in the hippocampal neurons of CUMS-exposed mice were attenuated by miR−134 overexpression. (A) NeuN immunohistochemistry in the hippocampal CA1, CA3, and DG regions (Ai: scale bar = 200 μm; ai−aiii: scale bar = 50 μm). (B) Representative Golgi staining images of dendritic spines in the hippocampal CA1, CA3, and DG regions (scale bar = 10 μm). (C) Representative electron microscopic images of PSD length, PSD thickness, and the width of the synaptic cleft in the hippocampal CA1 and CA3 regions (Ci and Cii: scale bar = 200 μm; ci and cii: scale bar = 50 μm). Data are presented as means ± SEM (n=6). ∗p < 0.05 and ∗∗p < 0.01 were considered statistically significant