Fig. 1.

G-Rh2 effectively inhibits CRC cell growth with a nontoxic effect on colon fibroblast cells. (A) The chemical structure of 20 (S)-ginsenoside Rh2. (B) HCT15, HCT116, DLD1, and CCD-18Co cells were seeded into 96-well plates and exposed to a range of concentrations of G-Rh2 for 24 h; cell viability was then determined by the CCK-8 assay. (C) HCT15, HCT116, and DLD1 cells were exposed to G-Rh2 (0, 10, 20, and 40 μM) for 0, 24, 48, and 72 h, and cell viability was determined by the CCK-8 assay. (D) and (E) Anchorage-independent growth showing colonies formed in HCT15, HCT116, and DLD1 cells after treatment with different concentrations of G-Rh2 for 12 d. Scale bar, 250 μm. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 versus control.