Fig. 4.

G-Rh2 directly binds to Axl and inhibits the Axl signaling pathway in CRC cells. (A) p-Axl and Axl expression in CRC cells and CCD-18Co cells were detected by western blotting. (B) In vitro pull-down assays were performed, and the interaction between G-Rh2 and Axl was confirmed by western blotting. (C) The effects of G-Rh2 on Axl signaling pathway protein expression in CRC cells were determined by western blotting. (D) Immunofluorescence staining of the Axl expression in HCT15 and HCT116 cells treated with G-Rh2 for 48 h (scale bar, 128 μm). (E) Statistical results of the fluorescence intensity of Axl protein. ∗P < 0.05, ∗∗P < 0.01 versus control.