Fig. 5.

Axl knockdown inhibits CRC cell growth in vitro and in vivo. (A) Axl knockdown in HCT116 cells was detected by the western blotting. (B) Cell viability in Axl knockdown HCT116 cells was determined by the CCK-8 assay. (C) and (D) Migration and invasion ability of Axl knockdown HCT116 cells (scale bar, 128 μm). (E) and (F) Flow cytometry analysis of the cell cycle in Axl knockdown HCT116 cells. (G) and (H) Colony formation assay in Axl knockdown HCT116 cells treated with 0, 20, and 40 μM of G-Rh2 (scale bar, 250 μm). (I) Tumor volume. (J) Tumor weight. (K) Images of xenograft tumors. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 versus control.