Figure 4.

Prediction of miRNAs that target the 3’-UTR of rat Egfr.

(A) Venn diagram of predicted miRNAs targeting the 3’-UTR of rat Egfr using three programs and supported by experimentally validated data from the Tarbase database (indicated by *). Red numbers indicate miRNAs that were predicted by at least two programs or supported by experimental evidence. Fifteen miRNAs supported by three programs are shown in the dashed rectangle. (B) Expression of 25 candidate miRNAs that target Egfr in silico that were predicted based on the spinal cord development dataset. Boxed text indicates that miRNAs were reported to inhibit cell proliferation in humans or mice. MiRNAs with significant negative correlations (P < 0.05) with Egfr expression are shown. (C) Phylogenetic analysis of the let-7 family in humans, mice, and rats. Seed sequences of let-7c, 7b, 7d, 7f, and 7e are highlighted with a red line.