Fig. 1.

Two dimensional GC-FID chromatogram of β-sitosterol standard and KGC11o. Analysis of KGC11o was performed using an DB-5 capillary column (30 × 0.32 mm, 0.25 μm; Agilent Technologies, Santa Clara, CA, USA) in a GC-FID (6890 system, Agilent Technology, USA). Samples were injected into the column and run in split mode (Split ratio = 10 : 1). The nitrogen carrier gas was programmed to maintain a constant flow rate of 2.0 mL/min. Oven temperature was initially 200 °C for 1min and then finally raised to 300 °C at 5 °C/min and maintained at 300 °C for 14 min.