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. 2022 Mar 31;17(5):1198–1214. doi: 10.1016/j.stemcr.2022.02.020

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Combined loss of Pcgf1/3/5/6 unleash precocious differentiation of ESCs

(A) Reference legend for cell lines used in this figure and in Figure S5.

(B) Western blot demonstrating changes in the levels of selected PcG proteins, pluripotency factors, germ layer markers, H2AK119ub1, and H3K27me3 in ESCs of indicated genotypes. Tubulin and H3 were used as loading controls.

(C) Top: phase-contrast images of ESC colonies cultured on a layer of MEFs. Middle and bottom: representative images of AP staining of ESC colonies of indicated genotypes cultured together with a feeder layer of MEFs (middle) or on gelatin (bottom). Scale bar, 100 μm.

(D) H&E staining of teratoma sections revealed cells from various cell lineages including gut epithelium (endoderm), cartilage (mesoderm) and neural rosette (ectoderm). Shown is a representative of three injected mice. Scale bars, 50 μm.