FIGURE 8.

Quantification of phytohormones in spruce bark before and after Endoconidiophora polonica infection. The concentration of abscisic acid (upper left panel), indole-3-acetic acid (upper right panel), jasmonic acid (lower left panel), and the (+)-7 jasmonic acid-isoleucine conjugate (lower right panel) were quantified by LC-MS/MS analysis from bark tissue harvested before and 14 days after infection with E. polonica. Trees investigated: (1) were from a stand with a history of previous bark beetle attack, but no current attack (PA), (2) were under current attack, but had no previous history of attack (CA), or (3) had no previous nor current attack (NA). Data are means ± SD of measurements from 6 to 12 biological replicates; exact numbers are given in the Section “Materials and Methods.” Statistical values for individual compounds are given in Supplementary Table 9.